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optimem  (Mirus Bio)


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    Structured Review

    Mirus Bio optimem
    Optimem, supplied by Mirus Bio, used in various techniques. Bioz Stars score: 99/100, based on 8554 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/optimem/TransIT+-LT1/pm41997112-299-11-3
    Average 99 stars, based on 8554 article reviews
    optimem - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Template-assisted covalent modification underlies activity of covalent molecular glues.
    Article Snippet: The next day, a packaging mix including 1.5 μg of psPAX2, 0.15 μg of pVSV-G and 1.5 μg of transgene plasmid was prepared in 37.5 μl of OptiMEM (Thermo Fisher Scientific). .. This mix was combined with 9 μl of TransIT-LT1 (Mirus) and 15 μl of OptiMEM, incubated for 30 min at room temperature and then applied dropwise to cells. ..

    Transfection:

    Article Title: Proteolysis of the Docking Protein HEF1 and Implications for Focal Adhesion Dynamics
    Article Snippet: .. Cells were then transfected with plasmid DNA (at a concentration of 0.1 μg/μl) in OptiMEM (Mirus) as described by the manufacturer. ..

    Plasmid Preparation:

    Article Title: Proteolysis of the Docking Protein HEF1 and Implications for Focal Adhesion Dynamics
    Article Snippet: .. Cells were then transfected with plasmid DNA (at a concentration of 0.1 μg/μl) in OptiMEM (Mirus) as described by the manufacturer. ..

    Concentration Assay:

    Article Title: Proteolysis of the Docking Protein HEF1 and Implications for Focal Adhesion Dynamics
    Article Snippet: .. Cells were then transfected with plasmid DNA (at a concentration of 0.1 μg/μl) in OptiMEM (Mirus) as described by the manufacturer. ..

    other:

    Article Title: mTORC1 activity suppresses ferroptosis through a SCARB1-dependent HDL-tocopherol uptake pathway.
    Article Snippet: Briefly, forward and reverse complement primers comprising the protospacer sequence of each sgRNA and overhangs complementary to the target backbone were annealed by pre-incubation at 37 ◦ C for 30 minutes in the presence of T4 polynucleotide kinase (NEB # M0201) followed by incubation at 95 ◦ C for 5 minutes and then ramp down to 25 ◦ C at 5 ◦ C/minute.

    Article Title: De novo recovery of Ghana virus, an African bat Henipavirus, reveals differential tropism and attenuated pathogenicity compared to Nipah virus
    Article Snippet: 21 mL of Mirus TransIT-LT1 were then added to the transfection reaction and gently mixed.



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